As revealed by the M.E.N. Matty Dewhurst posted that he had scooped £231.59 from an accumulator that included a victory for QPR, who were playing against his employers Bolton Wanderers on Tuesday night.
In a conversation with a pal the Academy fitness and conditioning coach, 23, posted on the site he revealed he had landed his winnings after betting £6 on the outcome of nine matches.
The Championship club, who lost 2-1, launched an investigation but it is understood Mr Dewhurst, a former Little Lever High pupil, offered his resignation and handed over a letter of apology.
Both were accepted.
There is no suggestion that Mr Dewhurst tried to influence the result but in light of the match-fixing allegations that have rocked football his actions were viewed as unwise.
FA rules prohibit all club staff from gambling on any match the club participates in.
Mr Dewhurst, who is from Bolton posted a status update revealing his delight at winning the bet.
"Derby County, 0-2 at home to Yeovil, come back to win 3-2 in the last minute to win me £230 for my accumulator!!. Buzzing!!," it read.
He was then told by a friend that he had trumped his own bet and responded by brandishing further details of his own wager.
"I had a few teams on to be fair, derby, QPR, yoounited (Manchester United), Reading, Real Madrid, Swansea, Wolves, Chesterfield and Norwich Newcastle draw, £6 for £231.59 back," he added.
On the same Facebook account Mr Dewhurst lists his job as Academy U9s - U16s, International Academy Strength and Conditioning Coach at the Reebok club.
Bolton have lost their last three games, one of which was a 7-1 hammering at Reading.
Mr Dewhurst's Facebook account could not be viewed tonight (Thursday).